Chem Biodivers. 2025 Apr 3:e202500531. doi: 10.1002/cbdv.202500531. Online ahead of print.
ABSTRACT
Cinnamomi Ramulus-Puerariae Lobatae Radix (CR-PLR) possesses antidiabetic macrovascular pathology pharmacological effects, but its pharmacological mechanism of action remains unclear. A total of 197 CR-PLR targets were identified with 1015 diabetic macrovascular disease-specific targets through network pharmacological analysis, and 55 intersecting targets were found. We screened five main active ingredients: Puerarin, 3'-Methoxydaidzein, Cinnamaldehyde, Cinnamic acid and Formononetin. The GO and KEGG analyses showed that CR-PLR may ameliorate diabetic macrovascular disease through the AGE-RAGE signaling pathway. In vivo experiments verified that CR-PLR significantly reduced body weight and blood glucose, improved vascular function, and attenuated inflammatory responses (e.g., IL-6, IL-4, etc.) and lipid metabolism abnormalities (e.g., TG, TC, LDL, HDL), which was corroborated by the results of immunofluorescence experiments. Western Blot experiments confirmed its action through the AGE-RAGE pathway. This study may provide an optimized method for elucidating the pharmacological mechanism of CR-PLR and a new drug candidate for the treatment of diabetic macrovascular disease.
PMID:40178178 | DOI:10.1002/cbdv.202500531